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New comment to be added:
Sable Energy Balance and Max Test 12/29/14 #18483
Energy expenditure measured by indirect calorimetry in the Promethion by Sable
Systems. Activity, food intake and water intake are also continuously measured.
Measurements were taken for 5 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.
Then energy expenditure measured by indirect calorimetry in the Oxymax by
Columbus Instruments. Mice were trained to run on the treadmill by running them
on a treadmill for 10 minutes at 10 m/min 2 to 3 days prior to the experiment.
On the day of the experiment mice were placed in the treadmill attached to the
Oxymax. The treadmill was turned off for 30 minutes to take readings of the
animal rested. Mice were then run on a treadmill at gradually increasing speeds
until exhaustion. When the mice became exhausted the treadmill was turned off
and recordings were taken for another 30 minutes. Speeds were increased by 4
m/min every 3 minutes, with the starting speed set to 10 m/min.
Sable run 1 is the Sable run, Sable run 2 is the treadmill run.
Male WT (C56BL/6J <+/+> ) and SIRT3 KO (C57BL/6J-Sirt3
Mice were fed a 60% kcal High Fat Diet (F3282 BioServ).
Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure, feeding behaviors, and different
Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.
day(s) post-natal (d)
DOWNLOAD ALL DATA (.csv)
Curation Info (# flags)
Name / Abbreviation
Experimental Factor: Experimental Group
This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor: Mouse Diet
Rodent Hig Fat Diet. Protein 20.%, Fat 35.5%, Fiber 0.0%, Ash 3.2%, Moisture <5.0%, Carbohydrate 36.3%. Ingredients: Lard, Casein, Dextrose, Sucrose, DL-Methionine, Choline Chloride, Vitamin Mix and Mineral Mix
Experimental Factor (Units)
Sable Run (RNum) (RNum)
Treadmill Speed (m/min)
Add / Edit
oxygen consumption (Light Period)
fat body mass
fat body mass
lean body mass
lean body mass
body water, free
heat (Light Period)
oxygen consumption (Dark Period)
carbon dioxide production Light Period
carbon dioxide production Dark Period
heat (Dark Period)
No documents found.
Mammalian Phenotype (MP) Terms
decreased total body fat amount
less than the normal total amount of connective tissue composed of fat cells within the entire body [MGI:csmith]
MMPC Search Results
decreased body fat mass
decreased physical bulk or volume of fat in the whole body [MGI:csmith]
MMPC Search Results
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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