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Experiment

Hyperinsulinemic euglycemic clamp order #10861
Summary Data Summary
Investigator Gettys, Thomas W
Description The UCP1 nulls and GCN2 nulls will allow us to test the involvement of these
respective genes as mediators (UCP1) or sensors (GCN2) of the physiological
phenotype produced by consuming a methionine restricted diet (e.g., #510071).

The WT, GCN2 null, and UCP1 null mice will be fed the Control diet for 1 wk
after arrival at Vanderbilt, and then half the animals of each genotype will be
randomized to receive the methionine restricted diet while the other half of the
animals of each genotype will continue to receive the control diet. The diets
will be provided ad libitum and fed to the mice for a total of 8-9 wks before
the conduct of hyperinsulinemic-euglycemic clamps on the mice.

Applicable research area(s): Diabetes, Obesity, Metabolism, Neurobiology
Status Completed
Public Release 3/11/2017
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals31
Experimental Conditions5
Catalog Items2
Curation Info (# flags)2
Phenotype Assays17
Phenotype Measurements8144
Histology Images0
Publications0
Phenotypes7


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
18
0
C57BL/6J-Ucp1-/-
0
13
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 methionine restricted diet (0.17%)
Methionine restricted diet - 0.17% methionine, glutamic acid 3.39%, energy content 3.812 kcal/g, 18.9% of calories from fat, 64.9% carbohydrate, and 14.8% a custom mix of L-amino acid.
 methionine restricted diet (0.86%)
Methionine restricted diet - 0.86% methionine, L-glutamic acid 2.7%, energy content 3.812 kcal/g, 18.9% of calories from fat, 64.9% carbohydrate, and 14.8% a custom mix of L-amino acid.



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