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Experiment

Sable Energy Balance #24532 3/16/18
Summary Data Summary
Investigator Brown, Jacob
Description We previously showed that the GLP1 receptor agonist, Exendin-4 (Ex4), reduces
body weight by acute reductions in food intake and chronic increases in energy
expenditure in obese mice. The mechanisms by which Ex4 increases energy
expenditure is not currently known. While weight loss by GLP1 receptor agonists
can be mediated by the brain, there is evidence that Ex4 can increase oxygen
consumption in skeletal muscle tissue directly. However, it is controversial
whether a canonical GLP1 receptor is expressed by skeletal muscle. Further, some
data suggest the possibility of another receptor in skeletal muscle through
which Ex4 can stimulate signaling. In this study, we will be using a global GLP1
receptor KO mouse to determine whether the canonical GLP1 receptor is necessary
for this Ex4-mediated increase in energy expenditure.

Energy expenditure measured by indirect calorimetry in the Promethion by Sable
Systems. Activity, food intake and water intake are also continuously measured.
Measurements were taken for 11 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.

Groups:
Male WT (C57BL/6J) mice.
Mice were fed a chow diet (LabDiet PicoLab Rodent Diet 5LOD).
Mice were treated with either PBS or Exendin-4.

Applicable research area(s): Metabolism, Obesity
Status Completed
Public Release 7/13/2020
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals16
Experimental Conditions3
Catalog Items1
Curation Info (# flags)1
Phenotype Assays14
Phenotype Measurements224
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
16
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Exendin-4
Exendin-4 is an incretin mimetic peptide, which is composed of 39 amino acids. It is an analog of glucagon-like peptide 1 (GLP-1), and an insulinotropic agent, with a long half-life. Exendin-4 has been used for the treatment of ob/ob mouse model t...
 PBS
Phosphate-buffered saline is a buffer salt solution containing disodium hydrogen phosphate and sodium chloride.

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...
Experimental Factor (Units)
Drug Administered
Experimental Group
Mouse Diet



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