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Experiment

Effect of antibiotic treatment on obesity-driven changes in gut permeability and
metabolic endotoxemia-strain comparison
Summary Data Summary
Investigator Murphy, William
Description Female Balb/c mice were fed either 10% LFD (D12450J) or 60% HFD (D12492) for 4
months. Female C57BL/6NTac mice were fed either 10% LFD (D12450J) or 60% HFD
(D12492) for 6 months. Animals on the high fat diet were divided into 2 groups :
control(water) or antibiotic- treated (Vancomycin, ampicillin, and neomycin
provided in drinking water for 14 days). Mice were euthanized and intestinal
tissues (ileum and colon) obtained for gut permeability testing (n=3-4 /group)
and plasma LBP assessment.
Status Completed
Public Release 2/28/2020
Animal Age Measured In: month(s) post-natal (m)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals21
Experimental Conditions7
Catalog Items2
Curation Info (# flags)2
Phenotype Assays3
Phenotype Measurements126
Histology Images0
Publications0
Documents1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6NTac
12
0
0
BALB/cAnNTac
9
0
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Ampicillin, Neomycin, Vancomycin
1g/L of Ampicillin, 1g/L of Neomycin, and 0.5g/L of Vancomycin mixed in water

Experimental Factor: Drug Delivery Route

 Drinking Water
The drug was delivered in the drinking water.

Experimental Factor: Drug Dose Units

 mg/ml
milligrams per milliliter

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Research Diets - D12450J
Rodent Diet with 10% fat, 70% carbohydrate, 20% protein
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Tissue Source

 colon
colon
 ileum
ileum



No documents found.

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