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Experiment

Hyperinsulinemic Euglycemic clamp order#22164
Summary Data Summary
Investigator Williams, Ashley
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT and Tg

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.

Metabolic disease is associated with muscle mitochondrial hyperacetylation.
These strains represent models of hyperacetylation and metabolic dysfunction.
Status Completed
Public Release 3/20/2020
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagAnimal Count
Data Analysis
TypeCount
Animals39
Experimental Conditions5
Catalog Items1
Curation Info (# flags)1
Phenotype Assays16
Phenotype Measurements9553
Histology Images0
Publications0
Phenotypes9


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
17
0
Cratfl Sirt3L2 MCK-cre DKO
0
10
0
Sirt3L2 MCK-cre
0
12
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Research Diets - D03021303i
High-fat diet with additional sucrose: 45 kcal% Fat and 25 kcal% Sucrose Special notes: Diet is irradiated with 10-20 kGy gamma-irradiation.



No documents found.


Mammalian Phenotype (MP) Terms

MPTermIDMPTermNameDefinitionOther Results
MP:0001260increased body weight
greater than normal average weight [J:33400]
MMPC Search Results
MP:0003926decreased cellular glucose uptake
reduced ability of a cell to take in glucose from the environment [RGD:cur]
MMPC Search Results
MP:0003927increased cellular glucose import
increased ability of a cell to take in glucose from the environment [RGD:cur]
MMPC Search Results
MP:0005559increased circulating glucose level
greater than the normal concentration in the blood of this major monosaccharide of the body; it is an important energy source [MGI:cwg| RGD:cur]
MMPC Search Results
MP:0005560decreased circulating glucose level
less than the normal concentration in the blood of this major monosaccharide of the body; it is an important energy source [MGI:cwg| RGD:cur]
MMPC Search Results
MP:0013278decreased fasting circulating glucose level
reduction in the amount of glucose in the blood at some defined time point after eating compared to controls [MGI:Armida_Di_Fenza]
MMPC Search Results
MP:0013279increased fasting circulating glucose level
increase in the amount of glucose in the blood at some defined time point after eating compared to controls [MGI:Armida_Di_Fenza]
MMPC Search Results
MP:0030020decreased cardiac cell glucose uptake
reduced ability of the cells of the heart to take in glucose [MGI:anna]
MMPC Search Results
MP:0030022decreased muscle cell glucose uptake
reduced ability of muscle cells to take in glucose [MGI:anna]
MMPC Search Results

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