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Experiment

Hyperinsulinemic Hyperglycemic clamp order #21836 Oct 2016
Summary Data Summary
Investigator Barish, Grant
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups:

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.

KO strain appears to be glucose tolerant

Applicable research area(s): Diabetes, Metabolism
Status Completed
Public Release 4/20/2021
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagAnimal CountExperiment Description
Data Analysis
TypeCount
Animals21
Experimental Conditions4
Catalog Items2
Curation Info (# flags)2
Phenotype Assays4
Phenotype Measurements987
Histology Images0
Publications0
Phenotypes1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6-Bcl6tm1.1Bar
0
9
0
C57BL/6-Bcl6tm1.1Bar Tg(Alb-Cre)
0
12
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...



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