mmpc-logo
twitter-logo @NationalMMPC
| Create Account | login
Experiment

Hyperinsulinemic clamp Order #18576 ,January 2016
Summary Data Summary
Investigator Robinson, Matthew
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups:

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.
Status Completed
Public Release 4/20/2018
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal CountExperiment Description
Data Analysis
TypeCount
Animals20
Experimental Conditions5
Catalog Items4
Curation Info (# flags)3
Phenotype Assays17
Phenotype Measurements5016
Histology Images0
Publications0
Phenotypes6


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
20
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Research Diet - D12450J
Rodent Diet with 10% kcal% fat. The fat comes from Lard and Soybean Oil
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat



No documents found.

Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.